Nadina Erill¹, Anna Colomer¹, Miquel Calvo², August Vidal³, Ruth Román^1, Montse Verdú³, Carlos Cordón-Cardó⁴, Xavier Puig^1,3.

¹BIOPAT, Grup Assistència, Statistics Department, ²Universitat de Barcelona, ³HISTOPAT Laboratoris, Barcelona, and ⁴Division of Molecular Pathology,  Memorial Sloan-Kettering Cancer Center,  New York.

Chromosome 18q allelic loss has been reported to have prognostic significance in stage II colorectal carcinoma. We have developed a fluorescent multiplex polymerase chain reaction assay to analyze five microsatellite markers (D18S55, D18S58, D18S61, D18S64 and D18S69) for allelic loss determination at the long […]

Anna Colomer¹, Nadina Erill¹, August Vidal², Miquel Calvo^3, Ruth Román¹, Montse Verdú^2, Carlos Cordon-Cardo⁴, Xavier Puig ^1,2.

¹BIOPAT, Grup Assistència, Barcelona; ²HISTOPAT Laboratoris, Barcelona; ³Statistics Department of the Universitat de Barcelona, Barcelona and ⁴Division of Molecular Pathology, Memorial Sloan-Kettering Cancer Center, New York.

High-frequency microsatellite instability has been reported to be associated with good prognosis in colorectal adenocarcinoma. However, methods to assess microsatellite instability (MIN) are based on genetic assays, and are not ideally suited to […]

Anna Colomer ¹, Nadina Erill ^1, August Vidal ², Ruth Román ¹, Montse Verdú ^2, Carlos Cordon-Cardo ³, Xavier Puig ^1,2.

¹BIOPAT, Grup Assistència, Barcelona, Spain; ²HISTOPAT Laboratoris, Barcelona, Spain; ³Division of Molecular Pathology, Memorial Sloan-Kettering Cancer Center, New York^.

The RAF gene family encodes RAS-regulated kinases that mediate cellular responses to growth signals. Mutations within the BRAF gene occur in about 10% of colorectal cancers, and are significantly associated to defective mismatch repair (MMR) due […]

Erill N¹, Colomer A¹, Calvo M³, Vidal A², Román R ¹, Verdú M², Cordon-Cardo C⁴, Puig X ^1,2.

¹BIOPAT, ²HISTOPAT Laboratoris, ³Statistics Dept. of the Universitat de Barcelona, Spain; and ⁴Division of Molecular Pathology, Memorial Sloan-Kettering Cancer Center, New York.

Chromosome 18q allelic loss (c18q LOH) seems to have prognostic significance in stage II colorectal carcinoma. In our laboratory c18q LOH was prospectively analyzed in a series of primary colorectal tumors using five markers (D18S55/58/61/64 and 69). Results of 207 PCR-based assays […]

Anna Colomer,¹ Nadina Erill,¹ Montse Verdú,² Ruth Roman,¹ August Vidal,^1,2 Carlos Cordon-Cardo,^1,3 Xavier Puig^1,2

¹BIOPAT, Grup Assistència, Barcelona; ²HISTOPAT Laboratoris, Barcelona; and ³Division of Molecular Pathology, Memorial Sloan-Kettering Cancer Center, New York.

Multiple studies using primary tumors have reported that alterations in p53 expression and detection of TP53 mutations are associated with clinical aggressiveness and poor response to specific therapies.  However, there is no general agreement regarding the optimal technical approach to the analysis of p53.  We have studied a series of […]