Detection of ALK and ROS1 rearrangements using Next Generation Sequencing in lung cancer patients: comparison between FISH, IHC and NGS

Sergi Clavé1,2, Natalia Rodon3, Lara Pijuan1, Alba Dalmases1,2, Olga Díaz3, Álvaro Taus4, Marta Lorenzo1,2, Pedro Rocha4, Ana M. Muñoz-Mármol5, Glòria Oliveras6, Joaquim Bosch6, Blanca Espinet1,2, Beatriz Bellosillo1,2, Xavier Puig3, Edurne Arriola2,4, Marta Salido1,2.

1 Servei de Patologia, Hospital del Mar, Barcelona. 2 Programa de Recerca en Càncer, Institut Hospital del Mar d’Investigacions Mèdiques, Barcelona. 3 BIOPAT. Biopatologia Molecular SL, Grup Assistència, Barcelona. 4 Servei d’Oncologia, Hospital del Mar, Barcelona. 5 Servei d’Anatomia Patològica, Hospital Germans Trias i Pujol, Badalona. 6 Departament […]

2018-04-23T11:28:41+00:00

Secuenciación masiva vs estudios moleculares gen a gen. Resultados de una serie de validación en 263 genes de 51 pacientes con tumores sólidos.

Natalia Rodón1, Olga Diaz1, Ruth Román1, Montse Verdú1,2, Yessica No Garbarino1, Isabel Trias1,2, Melina Lozano1 y Xavier Puig1,2,3.

1BIOPAT. Biopatologia Molecular SL, Grup Assistència, Barcelona; 2Histopat Laboratoris, Barcelona 3Hospital de Barcelona, SCIAS, Grup Assistència, Barcelona.

Presentamos los resultados del estudio comparativo realizado en BIOPAT para valorar la implementación de una plataforma de secuenciación masiva en sustitución de los estudios convencionales, gen a gen, para la caracterización del perfil molecular en una serie que incluye 19 carcinomas colorrectales, 26 de pulmón, […]

2017-07-11T15:20:54+00:00

Carcinoma seroso peritoneal y adenocarcinoma mucinoso apendicular sincrónicos. Estudio inmunohistoquímico y molecular.

Trias Ia,b,c, Verdú Ma,b, Rodón Nb, Román Rb, Orellana Ra, Barrios Pc y Puig Xa,b,c

a Histopat Laboratoris, b Biopat, Biopatología Molecular S.L., SCIAS, Grup Assistència, Barcelona, España, c Hospital de Barcelona, SCIAS, Grup Assistència, Barcelona, España.

Abstract

A 60 year old female with a family history of ovarian and breast cancer underwent a follow-up laparoscopic exploration which revealed peritoneal implants and mucinous deposits on the appendicular surface. The peritoneal implants were diagnosed as serous carcinoma with a characteristic immunophenotype, while the mucinous […]

2017-01-08T23:27:52+00:00

Synchronous serous peritoneal carcinoma and infiltrating mucinous appendicular adenocarcinoma. Immunohistochemical and molecular study.

Trias Isabel1,2,3, Verdú Montse1,2, Rodón Natalia2, Orellana Ruth1, Barrios Pedro3, Román Ruth2, García-Peláez Beatriz2, Díaz Olga2, Puig Xavier 1,2,3

1 Histopat Laboratoris, 2 Biopat, biopatología molecular SL, 3 Hospital de Barcelona. Barcelona, Spain (SCIAS), Grup Assistència, Barcelona (Spain).

 

Objective: To demonstrate the value of molecular studies in daily diagnosis.

Methods: Case report. 67y old woman had increased CA125, CA53, normal CEA and ascites with peritoneal carcinomatosis. Laparoscopic exploration with biopsies demonstrated mucinous perforated appendicular tumor with extra-appendicular acellular mucin and serous carcinoma peritoneal […]

2015-09-16T15:03:54+00:00

Cross-reactivity of EGFR L858R mutation-specific antibody in HER2 positive gastric adenocarcinomas

Verdu Ma, b, Trias Ia,b,c, Roman Rb,  Rodon Nb, Pubill Ca, Puig Xa,b,c

a Histopat Laboratoris, Barcelona, Spain. b BIOPAT, Biopatologia Molecular, Grup Assistencia, Barcelona, Spain. c Hospital de Barcelona, SCIAS, Grup Assistencia, Barcelona, Spain

 

Abstract:

Recently, the study of the EGFR mutations in lung cancer has become mandatory due to their usefulness in target therapy. Lately, the use of specific antibodies to study themost prevalent of these mutations has been introduced. The newly published discovery of the existence of a cross-reaction between […]

2015-03-01T20:20:57+00:00